Tissue engineering is defined by some characteristics such as biocompatibility, biodegradability, low immunogenicity, bio absorbability, nontoxicity, rejuvenation, and restoration properties. However, 3D-Scaffold are used having these characteristics with physical and chemical consideration. In this study a three-dimension scaffold was synthesized containing chitosan, Nanohydroxyapatite as an osteoconductive material, AgNO3 as an antiviral as well as anticancer therapeutic agent, and vancomycin that is considered for treating a number of bacterial infections. The behavior of the synthesized scaffold as a drug release material was investigated. Scaffolds were synthesized by freeze drying technique and characterized by scanning electron microscopy (SEM), Fourier transform infra-red spectroscopy, antibiogram method for In vitro tests and UV absorption device for evaluating the drug release behavior. It was found that drug delivery of scaffolds depended on the HA and AgNO3 content whereas HA resulted in more drug release and AgNO3 postponed the vancomycin release after about 1000 h examination. Moreover, the diagram of drug delivery system demonstrated the predictable release manner of vancomycin in scaffolds. Results of drug delivery tests indicated that AgNO3 have controllable efficacy on sustain delivery and with surpassing percentage of Ag, release of vancomycin have been extended more than 1 month with 70% delivery of drug.